Allow the test cassette, specimen, buffer, and/or controls to equilibrate to room temperature (15-30 °C) prior to testing.
1.Remove the test cassette from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.
2. Collect swine’s fresh whole blood or separate serum or plasma from blood as soon as possible to avoid hemolysis. Only use clear, non-hemolyzed specimens. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, specimens should be kept below -20 °C.
3.Place the test cassette on a clean and level surface.
4.For serum and plasma: Hold the dropper vertically and draw the specimen up to the Fill Line (approximately 5 µL), and transfer the specimen to the specimen well (S) of the test cassette, wait for 8-10 seconds, then add 2 drops of buffer (approximately 80 µl) and start the timer. For whole blood: Hold the dropper vertically, draw the specimen above 1cm above the Fill Line, and transfer 1 drop of whole blood (approximately 10 µL) to the specimen well (S) of the test cassette, wait for 8-10 seconds, then add 2 drops of buffer (approximately 80 µL) and start the timer.
5. Read the result in 5-10 minutes. Do not interpret results after 15 minutes.
INTERPRETATION OF RESULTS
Positive: The presence of both C line and T line, regardless of T line being strong or faint.
Negative: Only clear C line appears.
Invalid: No colored line appears in C line region, regardless of T line’s appearance.
